నైరూప్య

Effect of dietary mannanoligosaccharide on intestinal micro biota and immune parameters of Asian seabass (Lates calcarifer) juveniles.

S Syed Raffic Aliab, K Ambasankara, P Ezhil Praveenaa

Asixty days feeding experiment was carried out to evaluate effect of dietary Mannanoligosaccharide (MOS) on gut micro biota and immune parameters of Asian sea bass (Lates calcarifer) juveniles with mean initial body weight of (8.13 ± 0.06 g) were allocated in to 15 tanks with three replicates for each treatment with each replicate containing 15 animals. Experimental diets were prepared to contain (40% protein and 8% lipid) by supplementing MOS at four different concentrations (control 0%, 0.5%, 1%, 1.5% and 2%) levels in the diet of Asian sea bass. At the end of the experiment the results of serum immune parameters showed significant (p<0.05) differences in lysozyme activity, Alternative complement pathway activity, superoxide dismutase and nitro blue tetrazolium assay between control and MOS supplemented diets. PCR and DGGE analysis revealed that intestinal samples from MOS supplemented diet remain unaffected was failed to increase the intestinal microbial diversity and species richness. The results revealed that dietary MOS at 1.5% to 2% exerted a greater effect in immune parameters.