నైరూప్య

Histamine induced apoptosis in primary-cultured glioma

Bing Li, Chang Fei, Jian Zhang, Feng Guo, Aigang Sun, Linchun Huan, Shouzhong Guo, Yuhai Liu, Mei Shao, Yuquan Jiang

The study aim was to observe the apoptosis induction effects of histamine towards the Primary Cultured Human Glioma Cells (PCHGCs), and to discuss the relationships between the histamine-induced PCHGCs apoptosis and Ca2+ influx. The fresh glioma tissues obtained from the surgery were added with 10% foetal calf serum (FBS) containing 1640 nutrient solution for the cultivation. The cells in good conditions were added into 0.01 mg/ml, 0.5 mg/ml and 0.1 mg/ml histamine media for the primary cell cultivation. The Confocal Laser Scanning Microscope (CLSM) was used to detect the intracellular free Ca2+ concentration, and the Flow Cytometry (FCM) was used to detect the cell apoptosis rate under the effects of different histamine concentrations. PCHGCs that were cultured with 0.5 mg/ml and 0.1 mg/ml histamine solutions had the significant increase of intracellular free Ca2+ concentrations, which induced the apoptosis. The apoptosis occurrence was related to the histamine amount and the cultivation period in a certain extent. Histamine induced the PCHGCs apoptosis by increasing the concentration of intracellular free Ca2+.