నైరూప్య

Isolation and identification of Staphylococcus aureus from dairy farms in Bishoftu Town, Ethiopia

Sara Amanuel Bude, Abdi kidane Mengesha

Across-sectional study was conducted from February, 2020 to March, 2020 in selected dairy farms in Bishoftu Town to isolate and identify Staphylococcus aureus from milk samples, swab (teat swab, tank swab and bucket swab) samples. The samples were transported to microbiology laboratory and, isolation and identification of an organism was based on morphological, cultural and biochemical characteristics. Accordingly a total of 120 samples of them 58 milk sample, 58 polled teat swab sample, 2 polled tank swab sample and 2 polled bucket swab samples. Those milk samples and swab sample were collected and cultured parallel on both Blood agar and nutrient agar. Out of total samples 68/120 were well grown on both Medias and sub cultured on nutrient agar for farther identification using Primary and secondary biochemical tests. Accordingly, 68 milk samples were shown typical large, round, golden yellow colonies with hemolysis when grown on blood agar, round shaped grape like clusters are seen under microscope after stained with grams stain, and catalase positive Staphylococcus aureus with bubble formation is observed and typical yellow pigmentation on mannitol salt agar which is selective media for genus Staphylococcus. Finally, 68 (56.67%) Staphylococcus aureus were identified with coagulase test with the clumping result observed. Hence, implementing hygiene conditions, creation of awareness on control and prevention of subclinical mastitis in dairy farms and conducting drug sensitivity test for Staphylococcus aureus is recommended.